Table 1 EC50values of NRTN mutants and wild type.

The EC50 value represents the protein concentration required to obtain the half-maximal downstream signaling effect in TGW cells. Span of maximal signal of luciferase activity listed. Average of three experiments (Fig. 4D), four replicates per experiment. Reported SE is SEM.

ProteinEC50 (ng/ml)SEMax signal
(RLU)
SE
Wild-type
NRTN
2.11.2167,5006660
NRTN
(Y119A)
13.01.886,82017,290
NRTN
(E135S/
R139S)
3.21.3152,50010,190