Table 1 Antibodies used in this study.

Used dilutions for immunofluorescence (IF) and Western blotting (WB) and clone or catalog number (clone/order) are provided. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

AntibodySpeciesClone/orderSourceDilution IFDilution WB
FCHo2RabbitA303-396ABethyl1:200
FCHo2RabbitRA130H. McMahon1:2001:1000
FCHo1Mousesc-105351Santa Cruz1:100
β-ActinMouseac-15Sigma-Aldrich1:10,000
Clathrin heavy chainRabbitab21679Abcam1:2001:1000
Dynamin 1 + 2Mouse41BD Biosciences1:500
GAPDHRabbit1:5000
GFPMouse3E6Invitrogen A-111201:200
α-TubulinMouseDM1ASigma T96261:1000
Tf receptorMouseAb8598Abcam1:1000
Hip1RMouseSAB1406767Sigma1:1000
AP2MouseAP-6Homebrew from hybridoma1:1001:1000
HAMouseHA.11Babco1:2001:1000
HARat9E7Chromotek1:1000
Anti-rat AF647Goat712-605-153Jackson Imm. Res.1:200
Anti-mouse AF568GoatA11031Thermo1:200
Anti-mouse CF647Goat20281Biotium1:200
Anti-rabbit CF680Goat20067Biotium1:200