Table 1 Purification of Parapriacanthus luciferase.

Protein was calculated by the extract volume and concentration as determined by absorbance at 280 nm. rlu, relative light unit; HPLC, high-performance liquid chromatography.

Purification
method
Activity
(rlu)
Protein
(mg)
Specific
activity
(rlu/mg)
Purity
(fold)
Crude extract19714076261.20754751
HiTrap Q132804652.35565126275
Sephadex
G-75
120627591.82664614888
HPLC Mono Q
1 (fractions
21-27)
17184220.1710231190136
HPLC Mono Q
2 (fractions
35-42)
2090200.028709167115