| Amplified region | F/R | Sequence (5′ to 3′) |
| HBG promoters | F | GGAATGACTGAATCGGAACAAGG |
| R | CTGGCCTCACTGGATACTCT | |
| OT −197.1 | F | GTGGGTTATAGAAAGGGCAAGCTG |
| R | CCTTCCACTCAGATCTTGCCC | |
| OT −197.2 | F | CATCTAAGAGATTGGCAAAAATTATAGAGTCCCAAC |
| R | GTAATGTGATTGCTGGGTTGAGCC | |
| OT −197.3 | F | CTTCTCAAAAGAGCTAGGTGGCTG |
| R | GGGCCAGTACTTCCCTCTTTTTAG | |
| OT −197.4 | F | GATAGCACTGGCAACCTGTTTCTG |
| R | CTTCACTGTTTTGAGCCTCAGTTTCATTCC | |
| OT −197.5 | F | CCTCTTCTCTCCTCCCCTAAC |
| R | CCTGCTGTGTTCTAGGCTCTAAG | |
| OT −196.1 | F | GTGGGTTATAGAAAGGGCAAGCTG |
| R | CCTTCCACTCAGATCTTGCCC | |
| OT −196.2 | F | GCCGGGTTAAGGGCGTAGAA |
| R | CCCCTCAAACCCACTCTTAAAACC | |
| OT −196.3 | F | ATCACTTAAACAAGGATGCTCACCTCAGGTTC |
| R | CGGTCCAACCTGGAAATCTCTG | |
| OT −196.4 | F | CCCCTTTCATTTACTAACCAGGCATACC |
| R | GAGGGGCTTTTACTGGGCAATTG | |
| OT −196.6 | F | GTTATGGACTGTAGCAGGACAAGC |
| R | GTTGTGAGCCCTTAAAAGGCACAG | |
| OT −195.1 | F | ACAAACCATTGTGTGGGACTTTGGAGAC |
| R | CTATGCCATGTACTCTCCCTGAC | |
| OT −195.2 | F | CATGCCATGAAGGAAAATAAAGCAAGGTAAGAAG |
| R | CTAATACATTTCCTTACTTCATTCACGTCACTGC | |
| OT −115.1 | F | ATTGGGGGAGACAGACCCATG |
| R | CATTTTTATCCAGCTTGGCAGGGG |