| Amplified region | F/R | Sequence (5′ to 3′) |
| HBG A | F | TCAATGCAAATATCTGTCTGAAACG |
| R | CAAGGCTATTGGTCAAGGCAA | |
| HBG B | F | CAAATATCTGTCTGAAACGGTCCC |
| R | ACTCTAAGACTATTGGTCAAGTTTGC | |
| KLF1 | F | CCCAACCCAGGCAAATTG |
| R | GGGCTGGGAGTTGGGTCTT | |
| DEFB122 | F | TGTGGCTGGTCCTTGGGCTT |
| R | GTGGCTCCTGCCGTGACGAA |