Table 1 Summary of cKO loci targeting and mechanisms of donor DNA integrations.

Gene name, the names for cKO-targeted genes are indicated [official ID provided by MGI (Mouse Genome Informatics)]; No. of F0 selected animals, number of F0 founders selected to contain a positively targeted allele; No. of F1 analyzed animals, number of analyzed mice from the F1 generation; No. of F1 positive SC animals, number of mice with correct HDR-HDR single copy donor template integration; No. of F1 positive MC animals, number of mice with identified multiple integrated copies of donor template; (F0), multiple copy integration of donor DNA template was identified in F0 founders; template size/strandedness (ss-ds DNA), donor DNA template sizes and strandedness are indicated; mechanism, mechanism for donor DNA template integration as determined; nd - not determined.

Gene nameNo. of F0 selected
animals
No. of F1 analyzed
animals
No. of F1 positive
SC animals
No. of F1 positive
MC animals
Template size/
strandedness
(ss-ds DNA)
Mechanism
S100a8214 (No.6)
7 (No.11)
9 (No.6)
4 (No.11)
5
3
ssDNA
(PCR)
591 nt
HDR-HDR
NHEJ-HDR
NHEJ-NHEJ
Trek1221016dsDNA
1257 bp
HDR-NHEJ
NHEJ-HDR
Trek1628012ssDNA (PCR), 1257 ntHDR-HDR, NHEJ-HDR, HDR-NHEJ
Trek132603(F0)ssDNA
(IDT)
1286 nt
nd
Inf21134215ssDNA
(PCR)
711 nt
HDR-HDR
Trpc63225nddsDNA
880 bp
HDR-HDR
Trpc643452ssDNA
(PCR)
880 nt
HDR-HDR
Ccnd2146191(F0)dsDNA
1658 bp
HDR-HDR
Il4_5′LoxP18491930ssDNA
(PCR)
210 bp
HDR-HDR
Il4_flox44101(F0)ssDNA
(PCR)
1258 nt
NHEJ-HDR
Total F1: 63 (~43%)Total F1: 83 (~57%)