Protospacer adjacent motifs (PAMs) are highlighted in bold.
| gRNA | Target sequence (5′ to 3′) | Position (hg19) | Strand |
| AAVS1 | GGGGCCACTAGGGACAGGATTGG | chr19: 55627120–55627139 | − |
| −197 | ATTGAGATAGTGTGGGGAAGGGG | chr11: 5271268–5271287 (HBG1) | + |
| chr11: 5276192–5276211 (HBG2) | |||
| −196 | CATTGAGATAGTGTGGGGAAGGG | chr11: 5271267–5271286 (HBG1) | + |
| chr11: 5276191–5276210 (HBG2) | |||
| −195 | GCATTGAGATAGTGTGGGGAAGG | chr11: 5271266–5271285 (HBG1) | + |
| chr11: 5276190–5276209 (HBG2) | |||
| −158 | TATCTGTCTGAAACGGTCCCTGG | chr11: 5271243–5271262 (HBG1) | − |
| chr11: 5276167–5276186 (HBG2) | |||
| −152 | CCATGGGTGGAGTTTAGCCAGGG | chr11: 5271223–5271242 (HBG1) | + |
| chr11: 5276147–5276166 (HBG2) | |||
| −151 | CCCATGGGTGGAGTTTAGCCAGG | chr11: 5271222–5271241 (HBG1) | + |
| chr11: 5276146–5276165 (HBG2) | |||
| −115 | CTTGTCAAGGCTATTGGTCAAGG | chr11: 5271186–5271205 (HBG1) | + |
| chr11: 5276110–5276129 (HBG2) |