Table 1 gRNA target sequences.

Protospacer adjacent motifs (PAMs) are highlighted in bold.

gRNATarget sequence (5′ to 3′)Position (hg19)Strand
AAVS1GGGGCCACTAGGGACAGGATTGGchr19: 55627120–55627139
−197ATTGAGATAGTGTGGGGAAGGGGchr11: 5271268–5271287 (HBG1)+
chr11: 5276192–5276211 (HBG2)
−196CATTGAGATAGTGTGGGGAAGGGchr11: 5271267–5271286 (HBG1)+
chr11: 5276191–5276210 (HBG2)
−195GCATTGAGATAGTGTGGGGAAGGchr11: 5271266–5271285 (HBG1)+
chr11: 5276190–5276209 (HBG2)
−158TATCTGTCTGAAACGGTCCCTGGchr11: 5271243–5271262 (HBG1)
chr11: 5276167–5276186 (HBG2)
−152CCATGGGTGGAGTTTAGCCAGGGchr11: 5271223–5271242 (HBG1)+
chr11: 5276147–5276166 (HBG2)
−151CCCATGGGTGGAGTTTAGCCAGGchr11: 5271222–5271241 (HBG1)+
chr11: 5276146–5276165 (HBG2)
−115CTTGTCAAGGCTATTGGTCAAGGchr11: 5271186–5271205 (HBG1)+
chr11: 5276110–5276129 (HBG2)