Table 5 Primer sequences used for RT-qPCR. | Amplified region | F/R | Sequence (5′ to 3′) |
| HBA | F | CGGTCAACTTCAAGCTCCTAA |
| R | ACAGAAGCCAGGAACTTGTC |
| HBB | F | GCAAGGTGAACGTGGATGAAGT |
| R | TAACAGCATCAGGAGTGGACAGA |
| HBG1 + HBG2 | F | CCTGTCCTCTGCCTCTGCC |
| R | GGATTGCCAAAACGGTCAC |
| HBD | F | CAAGGGCACTTTTTCTCAG |
| R | AATTCCTTGCCAAAGTTGC |
| HBE | F | CTTTGGAAACCTGTCGTC |
| R | CTTGCCAAAGTGAGTAGC |
| GATA1 | F | GACAGGACAGGCCACTACCTATG |
| R | AGTACCTGCCCGTTTACTGACAA |
| ALAS2 | F | CAGCGCAATGTCAAGCAC |
| R | TAGATGCCATGCTTGGAGAG |
| BAND3 | F | ACCTCTCTCACCTCACCTTCTG |
| R | AACCTGTCTAGCAGTTGGTTGG |
| GAPDH | F | GAAGGTGAAGGTCGGAGT |
| R | GAAGATGGTGATGGGATTTC |