F, forward primer; R, reverse primer.
| Amplified region | F/R | Sequence (5′ to 3′) |
| HBG1 + HBG2 promoters | F | AAAAACGGCTGACAAAAGAAGTCCTGGTAT |
| R | ATAACCTCAGACGTTCCAGAAGCGAGTGTG | |
| HBG1 promoter | F | TACTGCGCTGAAACTGTGGC |
| R | GGCGTCTGGACTAGGAGCTTATTG | |
| HBG2 promoter | F | GCACTGAAACTGTTGCTTTATAGGAT |
| R | GGCGTCTGGACTAGGAGCTTATTG | |
| AAVS1 | F | CAGCACCAGGATCAGTGAAA |
| R | CTATGTCCACTTCAGGACAGCA |