Table 1 Crystallographic data and refinement statistics.

StructureAc-α-actin–
profilin–
NAA80ΔN
(CoA)
Ac-α-actin–
profilin–
NAA80ΔN
(AcCoA)
β/γ-actin–
profilin–
NAA80ΔN
(AnCoA)
Data collection
BeamlineMacCHESS F1MacCHESS F1MacCHESS F1
Date data
collection
11/27/201711/27/201711/27/2017
Wavelength (Å)0.9770.9770.977
Space groupC2221C2221C2221
Cell a, b, c (Å)104.5, 115.9,
132.5
103.8, 114.9,
132.3
104.4, 115.6,
132.2
Cell α, β, γ (°)90, 90, 9090, 90, 9090, 90, 90
Resolution (Å)50.0–2.0
(2.07–2.0)
50.0–2.9
(3.0–2.9)
50.0–2.5
(2.59–2.5)a
Rmerge (%)9.3 (57.6)17.3 (55.8)11.6 (56.3)
I/σI30.2 (4.5)24.3 (6.3)21.0 (4.0)
No. of unique
reflections
54,090 (5321)17,781 (1744)28,132 (2785)
Multiplicity26.3 (25.6)39.4 (40.3)13.1 (13.3)
Completeness
(%)
100.0 (100.0)100.0 (100.0)100.0 (100.0)
CC1/2b0.997 (0.947)0.994 (0.969)0.996 (0.974)
Wilson B-factor
2)
26.441.633
Resolution (Å)33.4–2.0 (2.08–2.0)28.7–2.9 (3.0–2.9)26.1–2.5 (2.59–2.5)
No. of unique
reflections
54,009 (5172)17,714 (1633)27,972 (2736)
Completeness
(%)
99.6 (97.0)99.1 (92.1)99.4 (98.2)
Rwork (%)15.9 (18.7)18.4 (22.1)17.8 (22.7)
Rfree (%)18.4 (22.8)23.9 (29.1)20.9 (28.02)
No. of atoms604655295598
Protein544953835259
Ligands137140135
Solvent (H2O)4606204
RMS deviations
Bond lengths (Å)0.0050.0030.003
Bond angles (°)0.860.690.72
B-factors (Å2)
Protein33.340.339.9
Solvent and
ligands
33.831.334.9
Ramachandran
(%)
Favored97.295.297.1
Outliers0.20.60.5
PDBc code6NBE6NAS6NBW

a, Numbers in parenthesis correspond to highest resolution shell; b, Pearson’s correlation coefficient; c, Protein Data Bank.