Table 1 List of reagents and resources.

ReagentsSourceIdentifier
Antibodies
AOX antiserum (custom raised in rabbit)21st Century Biochemicals, Marlborough, MA, USA(42)
HIF-1α (C-Term) polyclonal antibodyCayman Chemical10006421
Anti-beta actin antibody [mAbcam 8226]—loading
control (HRP)
Abcamab20272
Anti-rabbit IgG (H + L), HRP conjugatePromegaW4011
Anti-mouse IgG (H + L), HRP conjugatePromegaW4021
Lentiviral particles
Lentiviral Ciona intestinalis AOXThis paperN/A
Lentiviral catalytic inactive (mutant) AOXThis paper(20)
Chemicals
n-Propyl gallate (nPG, AOX inhibitor)Sigma-AldrichP3130
U46619 (prostaglandin H2/thromboxane A2
receptor agonist)
Enzo Life Sciences, Exeter, UKPG-023
Sodium chloride (NaCl)Sigma-Aldrich31434
Potassium chloride (KCl)Carl Roth, Karlsruhe, Germany5346.1
Calcium chloride (CaCl2)Carl RothCN93.1
Magnesium chloride (MgCl2)Carl RothKK36.1
Sodium dihydrogen phosphate dihydrate
(NaH2PO4·2H2O)
Merck Millipore, Darmstadt, Germany1063421000
Sodium hydrogen carbonate (NaHCO3)Carl Roth8551
d(+)-GlucoseCarl RothX997.1
l-Aspartic acid potassium salt (l-aspartate)Sigma-AldrichA6558
Adenosine 5′-triphosphate magnesium salt, ATPSigma-AldrichA9187
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-
tetraacetic acid (EGTA)
Sigma-Aldrich03777
Hepes (PUFFERAN®)Carl Roth9105.3
Potassium hydroxide (KOH)Carl Roth6751
1-Hydroxy-3-methoxycarbonyl
−2,2,5,5-tetramethylpyrrolidine (CMH spin
probe)
Noxygen, Elzach, GermanyNOX-02.2
Superoxide dismutase–polyethylene glycol (pSOD,
PEG-SOD)
Sigma-AldrichS9549
5,5′,6,6’-Tetrachloro-1,1′,3,3′-tetraethyl-
imidacarbocyanine iodide (JC-1)
ThermoFisher ScientificT3168
Penicillin-streptomycinGibco, ThermoFisher Scientific1507–063
Normocin—antimicrobial reagentInvivoGen, San Diego, USAant-nr-1
Assays
ZytoChem Plus (HRP) Polymer Bulk KitZytomed Systems GmbH, Berlin, GermanyPOLHRP-100
VECTOR NovaRED Peroxidase (HRP) Substrate KitVector Laboratories, Burlingame, USASK-4800
Enhanced chemiluminescence ECL Prime Western
Blotting System
GE HealthcareRPN2232
Cell Proliferation ELISA, BrdU (colorimetric) assaySigma-Aldrich11647229001
Primary cells
Mouse PASMC from precapillary pulmonary arterial
vessels
(30)N/A
Rat PASMC from precapillary pulmonary arterial
vessels
(45)N/A
Rodent models
Mouse: Rosa26-Aox (aka AoxRosa26)(10)N/A
Mouse: C57BL/6JCharles River, Sulzfeld, GermanyStrain Code 632
Rat: Sprague-Dawley (Crl:SD)Charles RiverStrain Code 400
Plasmid and vectors
pWPXL (constitutive lentiviral plasmid)Addgene, Boston, MA, USAPlasmid #12257
pMD2.G (envelope vector)AddgenePlasmid #12259
psPAX2 (packing vector)AddgenePlasmid #12260
Software and algorithms
Patchmaster softwareHEKA, Lambrecht, Germanyv2x90
IGOR Pro softwareWavemetrics, Lake Oswego, OR, USAversion 6.37
DatLab softwareOroboros Instruments, Innsbruck, AustriaDatLab 5
Prism softwareGraphPad Software; San Diego, CA, USAversion 8.2.0
Other
Multi Wire Myograph SystemDanish Myo Technology A/S, Aarhus, Denmark620 M
N2-balanced gas (21% O2, 5% CO2)Praxair, Danbury, CT, USA592091 (custom made)
N2-balanced gas (1% O2, 5% CO2)Praxair650329 (custom made)
Optical Oxygen Meter (FireStingO2)Pyro Science, Aachen, GermanyFSO2–2
Culture dishesGreiner Bio-One, Frickenhausen, Germany627860
Multi-line in-line solution heaterWarner Instruments, Hamden, CT, USASHM-8
Borosilicate glass capillary tubesSutter Instrument, Novato, CA, USABF150–86-10HP
DMZ universal electrode pullerZeitz, Martinsried, GermanyN/A
EPC 10 USB patch clamp amplifierHEKA895000
EMXmicro ESR spectrometerBruker Biospin GmbH, Rheinstetten, GermanyN/A
OROBOROS-2k oxygraphOroboros InstrumentsN/A
Media 199—Mammalian Cell CultureGibco, ThermoFisher Scientific31150–022
Vevo2100 high-resolution imaging system
equipped with a 40-MHz transducer
VisualSonics, Toronto, CanadaN/A
Raman spectrometer Shamrock 303iAndor, Technology, Belfast, UKSR-303i-A
LaserAltechna, Vilnius, LithuaniaDPSS 532
Raman filter cube with dichroic mirrorSemrock, Rochester, NY, USAzt532/640rpc
532-nm EdgeBasic best-value long-pass edge filterSemrockBLP01-532R-25
×40 magnification objectiveOlympus, Tokyo, JapanLUCPLFLN