Table 2 Vaccine-binding or HA-binding affinities of immunoglobulin G antibodies identified by RTX-NPS from day 7 post-vaccination plasmablasts.

Half-maximal effective concentration (EC50) values are reported as average ± SD (n ≥ 3). H3 A/Hong Kong/4801/2014 and B/Phuket/3073/2013 are components of the 2017-2018 Fluzone vaccine.

Monoclonal antibody
ID
Vaccine or HA strainEC50 (nM)
HT-AFluzone 2017-18Nonbinder*
HT-BH3 A/
Wisconsin/67/2005
0.43 ± 0.22
HT-BH3 A/New
York/55/2004
0.70 ± 0.36
HT-CB/Florida/4/20061.18 ± 0.63
HT-CB/Phuket/3073/20131.63 ± 0.32
HT-DH3 A/
Wisconsin/67/2005
0.76 ± 0.42
HT-DH3 A/New
York/55/2004
0.90 ± 0.42
HT-DH3 A/Hong
Kong/4801/2014
7.64 ± 2.94
HT-EFluzone 2017-180.57 ± 0.07

*HT-A bound to the Fluzone 2017-18 vaccine weakly.