Table 1
Thermocycling parameters used for the PCR amplification of the ERT2 fragment.
Cycle step
Temperature
Time
Cycles
Initial
denaturation
94°C
2 min
1
Denaturation
94°C
15 s
30
Annealing
58.5°C
45 s
Extension
72°C
45 s
Final extension
72°C
1 min
1
4°C
Hold