Table 1 NEPS and MLPL in vitro activities.

Summary of activities observed in in vitro assays with ISY and 8OG (Fig. 3 and figs. S16 to S20). Cyclisation refers to formation of nepetalactol isomers from the reactive ISY product; dehydrogenation refers to formation of nepetalactone isomers from corresponding nepetalactols. Ticks (✓), activity observed; question mark (?), possible activity, not verified; tilde (~), trace activity; double tick (✓✓), in vitro activity supported by in planta gene expression profiling data (only tested for N. mussinii).

EnzymeCyclizationDehydrogenation
cis-transcis-cistrans-ciscis-transcis-cistrans-cis
NmNEPS1?✓✓
NcNEPS1?
NmNEPS2
NcNEPS2
NmNEPS3✓✓~
NcNEPS3A
NmNEPS3B~
NmNEPS4✓✓?~
NcNEPS4?~
NmNEPS5?✓✓
NcNEPS5?
NmMLPL✓✓
NcMLPLA
NcMLPLB