Table 1 Statistics of the kinetic binding affinities between BHV-1 gD and nectin-1 IgV determined by SPR.

The fast-on/fast-off kinetic data are analyzed by the steady-state affinity model. The results from three independent experiments are listed.

Binding pairKD (M)Average KD (M)SD for KDModel
gD274 to HU-nectin-1-IgV7.575 × 10−77.008 × 10−70.676 × 10−7Steady-state affinity
6.260 × 10−7
7.188 × 10−7
gD274 to BO-nectin-1-IgV6.752 × 10−74.987 × 10−71.565 × 10−7Steady-state affinity
4.437 × 10−7
3.771 × 10−7
gD301 to HU-nectin-1-IgV7.648 × 10−78.792 × 10−71.008 × 10−7Steady-state affinity
9.183 × 10−7
9.546 × 10−7
gD301 to BO-nectin-1-IgV2.591 × 10−73.406 × 10−71.061 × 10−7Steady-state affinity
3.022 × 10−7
4.606 × 10−7
gD301-R188G to BO-nectin-1-IgV7.908 × 10−87.054 × 10−81.352 × 10−8Steady-state affinity
5.495 × 10−8
7.758 × 10−8
gD301-Y190G to BO-nectin-1-IgV8.067 × 10−77.502 × 10−71.648 × 10−7Steady-state affinity
8.974 × 10−7
5.646 × 10−7