Table 1 Association between rs3850997 and gastric cancer risk in GWAS and validation stages.

MAF, minor allele frequency; HWE, Hardy-Weinberg equilibrium. The covariates of age, sex, and study design were adjusted in GWAS stage; the covariates of age and sex were adjusted in the Nanjing-1, Nanjing-2, Yixing, Nantong, and Jilin stages; and sex was adjusted in both the Nanjing-3 and European stages.

StagesPopulationCases/controlsrs3850997 genotypes (TT/TG/GG)MAF (G allele)PHWEOR (95% CI)*P*
CasesControlsCase/control
GWASShanxi/Linxian1625/2100661/720/241706/1052/3420.371/0.4130.1310.83 (0.75–0.91)9.29 × 10−5
Validation-1Nanjing-11275/1436473/612/170489/715/2280.379/0.4090.2130.87 (0.78–0.98)1.73 × 10−2
Validation-2Nanjing-21021/1203398/424/163442/552/2060.381/0.4010.1360.92 (0.81–1.03)1.50 × 10−1
Validation-3Nanjing-3550/1155205/256/69400/555/1750.372/0.4000.4580.88 (0.76–1.03)1.09 × 10−1
Validation-4Yixing940/1061366/416/145339/518/1640.381/0.4140.1470.87 (0.76–0.99)3.02 × 10−2
Validation-5Nantong471/652167/218/65225/319/970.387/0.4000.3530.96 (0.80–1.14)6.18 × 10−1
Validation-6Jilin1096/1178402/510/155405/541/1990.384/0.4100.4280.89 (0.79–1.01)6.19 × 10−2
Combined6978/87850.87 (0.84–0.92)2.43 × 10−8
European254/283529/99/103222/1027/11860.660/0.6981.0000.74 (0.59–0.93)9.26 × 10−3
Combined7232/11,6200.87 (0.83–0.91)2.13 × 10−9

*The additive genetic model used in the logistic regression analysis was constructed to detect the association between rs3850997 and gastric cancer risk.

†A meta-analysis was performed for the combined group with Pheterogeneity = 0.820 for Asian populations and 0.669 for all populations.