Table 1 Data collection, processing, and refinement statistics.

Air-oxidized ACIII
(EMDB-0937)
(PDB 6LOE)
Dithionite-reduced
ACIII
(EMDB-0936)
(PDB 6LOD)
Data collection and processing
  Magnification22,50022,500
  Voltage (kV)300300
  Electron exposure (e/Å2)5960
  Defocus range (μm)1.2–3.31.0–3.0
  Pixel size (Å)1.041.04
  Symmetry imposedC1C1
  Initial particle images (no.)257,815488,581
  Final particle images (no.)177,489207,633
  Map resolution (Å)
FSC threshold
3.2
0.143
3.5
0.143
Refinement
  Model resolution (Å)3.33.5
    FSC threshold0.50.5
  Map sharpening B factor (Å2)−125−135
  Model composition
    Nonhydrogen atoms19,02419,024
    Protein residues2,3292,329
    Ligands1717
  RMSDs
    Bond lengths (Å)0.0250.025
    Bond angles (°)1.5481.420
  Validation
    MolProbity score1.952.20
    Clashscore8.5714.09
    Poor rotamers (%)0.420.21
  Ramachandran plot
    Favored (%)91.8490.03
    Allowed (%)8.169.97
    Disallowed (%)0.000.00