Table 1 Primary and secondary antibodies used for staining of specific compartments.

Stained
compartment
Antibodies/
binding proteins
SourceDilutionTreatment for antigen
retrieval
Cell stains
Neurons (HuCD)Mouse anti-HuCDMolecular Probes1:400tris buffer, 20 min, pH 8, 90°C
Oligodendrocytes (Olig2)Rabbit anti-Olig2Immuno-Biological
Laboratories
1:100Citrate buffer, 20 min, pH 6,
90°C
Microglia [(Iba 1) ionized
calcium binding adapter
molecule 1]
Rabbit anti-Iba1Wako1:800/
Astroglia [(GFAP) glial fibrillary
acidic protein]
Rabbit anti-GFAPDako1:500/
Astroglia [(GLT-1) glial
glutamate transporter 1]
Guinea pig anti-GLT-1Millipore1:500/
Myelin stains
Myelin basic protein (MBP)Rat anti-MBPAbcam1:400
Myelin oligodendrocytes
(CNPase)
Mouse anti-CNPaseBioLegend1:300Citrate buffer
20 min, pH 6, 90°C
Iron-proteins
FerritinGoat anti-FerritinSanta Cruz Biotechnology1:200/
TransferrinRabbit anti-TransferrinAbcam1:5000/