Table 1 Immune cell population in LN mice on days 2 and 7 after engraftment.

PBMCs from two patients with LN were engrafted in four NSG mice, and immune cell populations were profiled on days 2 and 7 by flow cytometry and are presented as percentages of total live cells. Naïve T cells were defined as CD3+CD45ROCD38FSCintermediate; Tm cells were defined as CD3+CD45RO+CD38FScintermediate; plasma cells were defined as CD3CD38+. Data were analyzed by Student’s t test.

% of total live cells
Day 2Day 7PN
SpleenCD4+ Tnaive0.17 ± 0.0022.18 ± 0.001<0.0014
CD4+ Tm0.04 ± 0.00011.24 ± 0.001<0.0014
CD8+ Tnaive0.21 ± 0.0011.84 ± 0.0001<0.0014
CD8+ Tm0.01 ± 0.00020.8 ± 0.011<0.0014
CD38+ plasma0.87 ± 0.00070.27 ± 0.001<0.0014
Bone marrowCD4+ T0.23 ± 0.0010.17 ± 0.003<0.0014
CD4+ Tm0.1 ± 0.0010.04 ± 0.0001<0.0014
CD8+ T0.23 ± 0.0010.14 ± 0.002<0.0014
CD8+ Tm0.03 ± 0.00020.001 ± 0.0001<0.0014
CD38+ plasma0.06 ± 0.00010.07 ± 0.0030.0164
LungCD4+ T1.16 ± 0.0071.4 ± 0.002<0.0014
CD4+ Tm0.25 ± 0.0080.24 ± 0.0009<0.0014
CD8+ T0.35 ± 0.00120.32 ± 0.001<0.0014
CD8+ Tm0.11 ± 0.0010.11 ± 0.0021.0004
CD38+ plasma0.12 ± 0.00090.22 ± 0.002<0.0014