Table 3 Measured values of δ11B and elemental ratios (B/Ca) within the skeletons of S. pistillata and corresponding reconstructed parameters of the calcifying fluid (pHcf, [CO32−]cf, and DICcf).

Measured values of δ11B and elemental ratios (B/Ca) within the skeletons of S. pistillata and corresponding reconstructed parameters of the calcifying fluid (pHcf, [CO32−]cf, and DICcf)..

Culture conditionsAnalytical results
pCO2swTpHswTankδ11Bc1 (±2 SD)δ11Bc2 (±2 SD)δ11Baverage (±2 SD)B/Ca (±2 SD)pHcf[CO32−]cf (±1 SE)DICcf (±1 SE)
(ppm)(°C)(total scale)(‰)(‰)(‰)(μmol/mol)(total scale)(μmol/kg)(μmol/kg)
Stylophora pistillata
466288.14223.20 ± 0.3023.21 ± 0.3023.20 ± 0.21470 ± 118.31888 ± 704464 ± 191
466288.142455 ± 11918 ± 714614 ± 194
466288.142459 ± 11909 ± 704584 ± 190
466288.14421.85 ± 0.3021.63 ± 0.3021.74 ± 0.21527 ± 118.21659 ± 624021 ± 166
466288.14123.95 ± 0.2623.95 ± 0.26432 ± 118.361050 ± 784815 ± 216
466288.14224.94 ± 0.2624.94 ± 0.26480 ± 118.431048 ± 704291 ± 181
499318.09218.09 ± 0.2618.09 ± 0.26378 ± 117.92553 ± 845283 ± 284
499318.09421.62 ± 0.0921.62 ± 0.09536 ± 118.21718 ± 543850 ± 163
499318.09123.48 ± 0.0923.48 ± 0.09513 ± 118.34934 ± 593968 ± 163
499318.091526 ± 11983 ± 634179 ± 184
499318.09420.93 ± 0.2620.93 ± 0.26411 ± 118.16851 ± 795046 ± 231
499318.09319.62 ± 0.0919.62 ± 0.09462 ± 118.06616 ± 634451 ± 203
499318.09122.04 ± 0.0922.04 ± 0.09557 ± 118.24728 ± 533692 ± 160
499318.091473 ± 11859 ± 624351 ± 187
925287.90223.26 ± 0.3623.26 ± 0.36419 ± 118.29980 ± 775097 ± 217
925287.90422.70 ± 0.2622.70 ± 0.26440 ± 118.25870 ± 674885 ± 207
925287.90122.25 ± 0.2622.25 ± 0.26451 ± 118.22800 ± 644770 ± 204
925287.90323.63 ± 0.2623.63 ± 0.26449 ± 118.32958 ± 674747 ± 200
925287.90223.32 ± 0.2623.32 ± 0.26485 ± 118.30855 ± 624403 ± 184
885317.97323.01 ± 0.2122.79 ± 0.2122.90 ± 0.158.19
885317.97123.21 ± 0.3923.21 ± 0.39417 ± 118.21920 ± 825038 ± 220
885317.97319.64 ± 0.2619.64 ± 0.26378 ± 117.91541 ± 795346 ± 286
885317.97419.76 ± 0.2619.76 ± 0.26595 ± 117.92353 ± 503408 ± 161
885317.97117.49 ± 0.2617.49 ± 0.26394 ± 117.65251 ± 734347 ± 474
885317.97223.27 ± 0.3923.27 ± 0.39417 ± 118.22929 ± 845036 ± 219
2807287.49322.11 ± 0.3922.11 ± 0.39384 ± 118.23983 ± 865622 ± 251
2807287.49321.90 ± 0.3921.90 ± 0.39377 ± 118.22968 ± 865726 ± 257
2807287.49421.51 ± 0.3921.51 ± 0.39357 ± 118.19969 ± 946046 ± 287
2807287.49421.40 ± 0.3921.40 ± 0.39355 ± 118.18958 ± 956095 ± 286
3194317.55421.74 ± 0.3921.74 ± 0.39410 ± 118.15819 ± 815101 ± 231
3194317.55222.75 ± 0.3922.75 ± 0.39425 ± 118.22903 ± 794895 ± 214
3194317.55322.62 ± 0.3622.62 ± 0.36408 ± 118.21928 ± 785106 ± 226
3194317.55322.05 ± 0.3622.05 ± 0.36419 ± 118.17836 ± 784984 ± 218
3194317.55119.15 ± 0.3919.15 ± 0.39342 ± 117.92605 ± 935888 ± 330
3194317.55222.07 ± 0.2822.14 ± 0.2822.10 ± 0.20455 ± 118.17773 ± 614585 ± 202

pCO2sw, seawater pCO2 of the culture treatment.

T, seawater temperature of the culture treatment.

pHsw, seawater pH of the culture treatment.

Tank, tank number where the sample was cultured.

δ11Bc1, first measurement of the boron isotopic composition of the sample, uncertainty determined on reproducibility of the AE121 standard (2 SD).

δ11Bc2, duplicate analysis of the boron isotopic composition of the sample, uncertainty determined on reproducibility of the AE121 standard (2 SD).

δ11Baverage, average of δ11Bc1 and δ11Bc2 or δ11Bc1 if only one measurement was performed. When two measurements were carried out uncertainty was calculated based on uncertainties of δ11Bc1: and δ11Bc2a = √ (Σiai)2)).

B/Ca measured for the sample. Uncertainty is based 2 SD on the reproducibility of the CamWuellestorfi (table S4).

pHcf calculated from δ11B using (8) based on Monte Carlo simulation.

[CO32−]cf calculated from B/Ca using (8).

DICcf calculated from δ11B and B/Ca using (8).

Uncertainties for [CO32−]cf and DICcf calculated using (8), Δ (1 SE) = √ (Σi(Δsysi+Δnonsysi)2), xuncertainty = Δnonsys (1 SE).Additional data not used in this study (Li/Ca, Mg/Ca and Sr/Ca) are presented in table S7 in the Supplementary Materials.