Table 2 Measured values of δ11B and elemental ratios (B/Ca) within the skeletons of P. damicornis and corresponding reconstructed parameters of the calcifying fluid (pHcf, [CO32−]cf, and DICcf).

Measured values of δ11B and elemental ratios (B/Ca) within the skeletons of P. damicornis and corresponding reconstructed parameters of the calcifying fluid (pHcf, [CO32−]cf, and DICcf)..

Culture conditionsAnalytical resultsCalculated parameters
pCO2swTpHswTankδ11Bc1 (±2 SD)δ11Bc2 (±2 SD)δ11Baverage (±2 SD)B/Ca (±2 SD)pHcf[CO32−]cf (±1 SE)DICcf (±1 SE)
(ppm)(°C)(total scale)(‰)(‰)(‰)(μmol/mol)(total scale)(μmol/kg)(μmol/kg)
Pocillopora damicornis
466288.14119.70 ± 0.1019.65 ± 0.1019.68 ± 0.07472 ± 118.04522 ± 664458 ± 199
466288.14221.52 ± 0.1021.63 ± 0.1021.58 ± 0.07545 ± 118.19621 ± 573891 ± 162
466288.14322.76 ± 0.1022.65 ± 0.1022.71 ± 0.07449 ± 118.28877 ± 704694 ± 206
466288.14423.67 ± 0.1123.68 ± 0.1123.68 ± 0.08492 ± 118.34894 ± 654240 ± 175
466288.14124.42 ± 0.3924.42 ± 0.39489 ± 118.39975 ± 764231 ± 178
466288.14219.01 ± 0.3919.01 ± 0.39460 ± 117.97463 ± 814513 ± 222
499318.09422.02 ± 0.2122.09 ± 0.2122.06 ± 0.29537 ± 118.25759 ± 613840 ± 157
499318.09422.38 ± 0.0922.38 ± 0.09537 ± 118.27789 ± 553834 ± 162
499318.09121.02 ± 0.0921.02 ± 0.09501 ± 118.17707 ± 574130 ± 176
499318.0922.01 ± 0.1122.02 ± 0.1122.01 ± 0.08500 ± 118.24809 ± 574126 ± 174
499318.09318.53 ± 0.1118.50 ± 0.1118.51 ± 0.08504 ± 117.96455 ± 584000 ± 188
499318.09415.56 ± 0.1115.53 ± 0.1115.55 ± 0.08470 ± 117.60184 ± 603459 ± 461
925287.90217.22 ± 0.1117.23 ± 0.1117.23 ± 0.08435 ± 117.73263 ± 584329 ± 339
925287.90119.78 ± 0.1119.63 ± 0.1119.71 ± 0.08558 ± 118.01422 ± 453807 ± 162
925287.90322.98 ± 0.2622.82 ± 0.2622.90 ± 0.18492 ± 118.27800 ± 554361 ± 178
925287.90222.25 ± 0.0922.25 ± 0.09491 ± 118.22735 ± 544383 ± 185
925287.90421.60 ± 0.0921.60 ± 0.09503 ± 118.17652 ± 544280 ± 178
925287.90121.69 ± 0.0921.69 ± 0.09619 ± 118.18538 ± 423482 ± 136
885317.97421.32 ± 0.0921.32 ± 0.09743 ± 118.07387 ± 382812 ± 114
885317.97318.94 ± 0.0918.94 ± 0.09474 ± 117.84356 ± 564141 ± 230
885317.97121.89 ± 0.2121.85 ± 0.2121.87 ± 0.158.11
885317.97321.88 ± 0.0921.88 ± 0.09592 ± 118.11533 ± 463550 ± 146
885317.97317.84 ± 0.1117.80 ± 0.1117.82 ± 0.08524 ± 117.70217 ± 503422 ± 286
885317.97121.47 ± 0.1121.46 ± 0.1121.46 ± 0.08441 ± 118.08668 ± 614751 ± 208
2807287.49122.59 ± 0.2622.53 ± 0.2622.56 ± 0.18460 ± 118.27865 ± 604673 ± 192
2807287.49422.60 ± 0.1122.35 ± 0.1122.48 ± 0.08466 ± 118.26845 ± 574613 ± 187
2807287.49321.19 ± 0.1121.24 ± 0.1121.21 ± 0.08422 ± 118.17782 ± 645118 ± 224
2807287.49222.69 ± 0.1122.57 ± 0.1122.63 ± 0.08496 ± 118.27811 ± 544339 ± 175
2807287.49221.48 ± 0.2121.48 ± 0.218.19
3194317.55422.17 ± 0.3922.17 ± 0.39485 ± 118.18734 ± 694300 ± 178
3194317.55220.47 ± 0.0920.47 ± 0.09486 ± 118.04557 ± 534266 ± 184
3194317.55315.61 ± 0.0915.61 ± 0.09538 ± 117.3974 ± 7x2276 ± 108
3194317.55416.06 ± 0.0916.06 ± 0.09518 ± 117.49113 ± 462774 ± 94
3194317.55114.60 ± 0.1114.59 ± 0.1114.60 ± 0.08449 ± 117.0214 ± 4x950 ± 175
3194317.55420.24 ± 0.1120.29 ± 0.1120.26 ± 0.08509 ± 118.02512 ± 504061 ± 180
3194317.55214.00 ± 0.1113.94 ± 0.1113.97 ± 0.08523 ± 116.38

pCO2sw, seawater pCO2 of the culture treatment.

T, seawater temperature of the culture treatment.

pHsw, seawater pH of the culture treatment.

Tank, tank number where the sample was cultured.

δ11Bc1, first measurement of the boron isotopic composition of the sample, uncertainty determined on reproducibility of the AE121 standard (2 SD).

δ11Bc2, duplicate analysis of the boron isotopic composition of the sample, uncertainty determined on reproducibility of the AE121 standard (2 SD).

δ11Baverage, average of δ11Bc1 and δ11Bc2 or δ11Bc1 if only one measurement was performed. When two measurements were carried out, uncertainty was calculated based on uncertainties of δ11Bc1 and δ11Bc2a = √ (Σiai)2)).

B/Ca measured for the sample. Uncertainty is calculated as 2 SD of repeated measurements of the CamWuellestorfi standard (table S4).

pHcf calculated from δ11B using (8) based on Monte Carlo simulation.

[CO32−]cf calculated from B/Ca using (8).

DICcf calculated from δ11B and B/Ca using (8).

Uncertainties for [CO32−]cf and DICcf calculated using (8), Δ (1 SE) = √ (Σi(Δsysi+Δnonsysi)2), xuncertainty = Δnonsys (1 SE).Additional data not used in this study (Li/Ca, Mg/Ca and Sr/Ca) are presented in table S7 in the Supplementary Materials.