Table 1 Measured and calculated seawater parameters (±SE) from the culturing experiments (S, salinity; T, temperature; pH (NBS scale); DIC, dissolved inorganic carbon; Alkalinity, total alkalinity; pH on total scale; [CO32−], carbonate ion; [HCO3], bicarbonate ion; [CO2], partial pressure of CO2; ΩA, saturation state of seawater with respect to aragonite).

ExperimentSeawater measured parameters
δ11Bsw*
(‰)
S
(psu)
T
(°C)
pH
(NBS scale)
DIC
(μmol/kg-seawater)
Alkalinity
(μmol/kg-seawater)
Exp1 (400 ppm, 28°C)41.02 ± 0.4934.9 ± 0.128.29 ± 0.018.27 ± 0.012480 ± 402915 ± 49
Exp2 (400 ppm, 31°C)40.17 ± 0.3335.6 ± 0.131.72 ± 0.068.24 ± 0.012350 ± 442774 ± 54
Exp3 (1000 ppm, 28°C)41.36 ± 0.1335.4 ± 0.127.88 ± 0.028.04 ± 0.012645 ± 442939 ± 46
Exp4 (1000 ppm, 31°C)41.93 ± 0.2036.0 ± 0.130.83 ± 0.028.12 ± 0.012755 ± 373083 ± 40
Exp5 (2800 ppm, 28°C)40.97 ± 0.1535.8 ± 0.128.17 ± 0.027.62 ± 0.012861 ± 822907 ± 81
Exp6 (2800 ppm, 31°C)41.35 ± 0.1235.7 ± 0.130.93 ± 0.027.69 ± 0.013223 ± 573290 ± 56
ExperimentSeawater calculated parameters
pCO2
(ppmv)
pH**
(total scale)
[CO32−]
(μmol/kg-seawater)
[HCO3]
(μmol/kg-seawater)
[CO2]
(μmol/kg-seawater)
ΩA
Exp1 (400 ppm, 28°C)466 ± 88.14 ± 0.01334 ± 92133 ± 3312.3 ± 0.25.38 ± 0.14
Exp2 (400 ppm, 31°C)499 ± 98.09 ± 0.01320 ± 112017 ± 3612.3 ± 0.35.20 ± 0.18
Exp3 (1000 ppm, 28°C)925 ± 157.90 ± 0.01217 ± 62424 ± 3524.6 ± 0.43.48 ± 0.10
Exp4 (1000 ppm, 31°C)885 ± 127.97 ± 0.01265 ± 42468 ± 3522.0 ± 0.44.28 ± 0.07
Exp5 (2800 ppm, 28°C)2807 ± 1207.49 ± 0.0190 ± 32697 ± 7774.3 ± 3.21.44 ± 0.04
Exp6 (2800 ppm, 31°C)3194 ± 1367.55 ± 0.01113 ± 43030 ± 5479.5 ± 3.41.83 ± 0.06

11Bsw-based average of δ11Bsw1 and δ11Bsw2 (see table S5) for each condition.

**pH on total scale recalculated on the basis of pH measured on NBS scale during experiment.